buf make

Subcommand Description

buf make calculates the amount of each chemical that is required to make a solution/buffer of a given volume.

Using Your Recipe Library

To make a solution using a previously defined recipe (see buf recipe), use buf make <volume> <recipe_name>. For example, to make 2L of a buffer named ‘wash’, (where ‘wash’ has already been defined with buf recipe -a ...), use buf make 2L wash. The volume of the buffer does not need to be specified only in litres, for example buf make 250mL wash would be acceptable as well.

Defining a Recipe as it is Made

Alternatively, one can define a solution on the spot, with buf make <volume> (<concentration> <chemical_name>).... For example, ‘buf make 0.5L 300mM NaCl 10% glycerol’. Note that in this case, the molar mass of NaCl must already be stored in your chemical library (see buf chemical).

A Note on Copying Tables

If one wishes to copy and paste a table outputted by buf make (for example, into a text file to print), make sure that one uses the font ‘New Courier’, in order for the table to be formatted properly.