buf make¶
Subcommand Description¶
buf make
calculates the amount of each chemical that is required to make a solution/buffer of a given volume.
Using Your Recipe Library¶
To make a solution using a previously defined recipe (see buf recipe), use buf make <volume> <recipe_name>
. For example, to make 2L of a buffer named ‘wash’, (where ‘wash’ has already been defined with buf recipe -a ...
), use buf make 2L wash
. The volume of the buffer does not need to be specified only in litres, for example buf make 250mL wash
would be acceptable as well.
Defining a Recipe as it is Made¶
Alternatively, one can define a solution on the spot, with buf make <volume> (<concentration> <chemical_name>)...
.
For example, ‘buf make 0.5L 300mM NaCl 10% glycerol’. Note that in this case, the molar mass of NaCl
must already be stored in your chemical library (see buf chemical).
A Note on Copying Tables¶
If one wishes to copy and paste a table outputted by buf make
(for example, into a text file to print), make sure that one uses the font ‘New Courier’, in order for the table to be formatted properly.