buf make ======== Subcommand Description ++++++++++++++++++++++ ``buf make`` calculates the amount of each chemical that is required \ to make a solution/buffer of a given volume. Using Your Recipe Library +++++++++++++++++++++++++ To make a solution using a previously defined recipe (see :doc:`buf recipe `), \ use ``buf make ``. For example, to make 2L of a buffer named 'wash', \ (where 'wash' has already been defined with ``buf recipe -a ...``), use ``buf make 2L wash``. The volume of the buffer \ does not need to be specified only in litres, for example ``buf make 250mL wash`` would be acceptable as well. Defining a Recipe as it is Made +++++++++++++++++++++++++++++++ Alternatively, one can define a solution on the spot, with ``buf make ( )...``. For example, 'buf make 0.5L 300mM NaCl 10% glycerol'. Note that in this case, the molar mass of NaCl must already be stored in your chemical library (see :doc:`buf chemical `). A Note on Copying Tables ++++++++++++++++++++++++ If one wishes to copy and paste a table outputted by ``buf make`` (for example, into a text file to print), \ make sure that one uses the font 'New Courier', in order for the table to be formatted properly.